So polymerase binds to the 5' end. And it moves towards the 3' end. If we say for the sake of this explanation that 5' is left and 3' is right... It is moving from left to right but can only add nucleotides to the right side of the strand.
So polymerase binds to the 5' end. And it moves towards the 3' end. If we say for the sake of this explanation that 5' is left and 3' is right... It is moving from left to right but can only add nucleotides to the right side of the strand.
Right so it attaches at the 5' and not the 3'? ( so where does the hydroxyl group come in?)
The polymerase, as explained above, moves left to right. As in 5' to 3'. However the nucleotides are added at the 3' end of the polymerase then the polymerase travels past this.
Edit: Basically, think of the end on the right of the polymerase as the 3' where it actively adding bases while chugging along. So while it's moving from the 5' to the 3', the right of the polymerase is always considered the the 3' end, where the bases are added.
The polymerase, as explained above, moves left to right. As in 5' to 3'. However the nucleotides are added at the 3' end of the polymerase then the polymerase travels past this.
Ohh okay, so it attached at the 3' and just traveled by it. It starts to assemble nucleotides from the 5' end towards the 3'? Thanks
Hello, In order to understand better how it adds nucleotides in the growing strand try and visualize the enzyme as a zipper that pries open in half the growing strand and walks on the dna template while adding nucleotides in the complementary bases along the '3 growing strand which is always from left to right.
Have a look at this below to visualize the process. Good luck on your exams!