The Student Room Group

Biology Help

Can anyone explain to me how the Benedict's test for non-reducing sugar works please? The procedure.
For the non-reducing sugar, you add 2cm3 of dilute hydrochloric acid (to 2cm3 of your sample); after having done this experiment once to confirm that there is no reducing sugar present.This hydrolyses the glycosidic bond; freeing the aldehyde and ketone groups from the non-reducing sugar.You will then heat in the water bath.Neutralise it with sodium hydrogen carbonate (test with litmus paper).Add 2cm3 of Benedict's solution to the heated sample; if it forms a coloured precipitate, a non-reducing sugar is present.
(edited 7 years ago)
Reply 2
Avatar for Abirella
Abirella
OP
14
Original post by Bigbosshead
For the non-reducing sugar, you add 2cm3 of dilute hydrochloric acid (to 2cm3 of your sample); after having done this experiment once to confirm that there is no reducing sugar present.This hydrolyses the glycosidic bond; freeing the aldehyde and ketone groups from the non-reducing sugar.You will then heat in the water bath.Neutralise it with sodium hydrogen carbonate (test with litmus paper).Add 2cm3 of Benedict's solution to the heated sample; if it forms a coloured precipitate, a non-reducing sugar is present.


Thanks :smile:


Posted from TSR Mobile

Quick Reply

Related discussions

Latest

Trending

Trending

Articles for you