here is my own question set:
(1) Tricholoma matsutake is a fungus that produces amylase as a secretion. Students decided to test different pieces of fungi at different pH to see how it would affect the action of the amylase. They were placed in a starch medium dyed with iodine.
(a) (i) Suggest how the amylase exits the fungal cells.
(ii) Another polysaccharide is chitin. This is in the cell walls of fungi. Explain why amylase hydrolyses the starch but not the chitin.
(b) The students note that after 60 minutes, there is a clear area at pH 6 and 8 whilst at pH values less than 6 or greater than 8 remained dark blue. The clear area was greatest at pH 8.
(i) Explain why at pH values above 8 there are no clear areas.
(ii) The teacher suggests a control experiment should be carried out. Suggest a suitable control experiment.
(iii) One student concludes that the optimum pH for the enzyme amylase is 8. Explain whether or not this is justified.
(c) State THREE factors that should have been constant during this investigation.