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Restriction mapping help

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  1. Offline

    I really dont understand restriction mapping. Ive looked at various textbooks, you tube videos...and i still cant comprehend it.

    Can somebody explain in the most simplest and easiest way (like a step to step guide) on how to perform this procedure?
  2. Offline

    Restriction mapping is used to create a map of a plasmid (with a known length of DNA and/or sequence) that shows the points slong the plasmid that certain enzymes witll cut it at.

    What happens is the purifyed plasmid is placed in a vessel with one type of enzyme.

    The enzyme then digests the plasmid and breaks it up into fragments.

    The fragment are the run along a electrophoresis gel and this then allows them to be measured against a guide to fins out how long each of the fragments are.

    If you know what base sequence the enzyme digests and the sequence and legthens of the fragments and this is done for many different enzymes you can make a map that looks like this

    It can help biologist to isolate particular genes in a plasmid to remove them
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    Here the pic
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    i know what it is...but i dont know how to do the calculations


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