[otrivine]

(Original post by Sparkzz)
Ok, i will give u a set of questions, however slow replies so i apologise in advance!!

1) Name three advantages of Immobilising enzymes (3 marks)
2) What is a clone? (2 marks)
3) Define vegetative propagation. (1 mark)
4) Define hemizygous (2 marks)
5) Why can't males inherit sex-linked conditions from their fathers? (2 marks)

10 marks.


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1) enzyme molecules do not mix freely with substrate so Purification costs are low
The enzyme molecules are more stable as they are proteceted by the enzyme matrix and enzymes are available for reuse.
2)its to produce genetically identical daughter cells and are same as their parent cells .mitosis
3)Refers to the individual producing new structures and these grow to a new individual but they are genetically the same
3)you mean homozygous right ?
4) Because of different chromosomes X on the male, heterozygous alleles, different gene loci

[ds4143]

Ohh yes I remember that now, thanks!

And thats good! except for the prophase thing sparkzz already mentioned...and additional info, independent assortment also happens at metaphase II

Got a question for me ?

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[otrivine]

(Original post by ds4143)
Ohh yes I remember that now, thanks!

And thats good! except for the prophase thing sparkzz already mentioned...and additional info, independent assortment also happens at metaphase II

Got a question for me ?

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thanks where did you go ? answer faster

ok
Describe the process of electrophoresis (4)

[Sparkzz]

(Original post by otrivine)
1) enzyme molecules do not mix freely with substrate so Purification costs are low
The enzyme molecules are more stable as they are proteceted by the enzyme matrix and enzymes are available for reuse.
2)its to produce genetically identical daughter cells and are same as their parent cells .mitosis
3)Refers to the individual producing new structures and these grow to a new individual but they are genetically the same
3)you mean homozygous right ?
4) Because of different chromosomes X on the male, heterozygous alleles, different gene loci

1) yes correct
2) yes correct
3) yes correct
4) hemizygous is when one allele is responsible for the characteristic. check page 24.
5) because the x chromosome is is inherited from the mother. therefore males only inherit the y chromosome from their father.


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[otrivine]

(Original post by Sparkzz)
1) yes correct
2) yes correct
3) yes correct
4) hemizygous is when one allele is responsible for the characteristic. check page 24.
5) because the x chromosome is is inherited from the mother. therefore males only inherit the y chromosome from their father.


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in my book page 24 is on F214?????????
are u using a different book cause in the ocr does not mention !?
more please
ok let me give you some as well so did i get 7/10 ?
1)Suggest Name 2 ways to treat sickle cell and what amino acid is replaced (3)
2)what do you understand by the term Restriction enzyme (1)

[ds4143]

1. Inexpensive as separation of the enzyme-substrate complex from the mixture is not needed. The enzyme becomes much more stable, therefore reducing the chances of it becoming denatured. Also it can be reused

2. An organism that is genetically identical to the organism that has been used to produce the clone. For example Binary fission, bacteria cells dividing asexually producing clones.

3. Is when a structure is taken from another organism and used to grow into another individual organism. -genetically identical to parent cells.

4. Hemizygous...Im not sure is it something to do with having one allele and not the other :/?

5. Because males ummm im not sure lol




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[DoctorVertigo]

I hate this paper. This is the one paper that's gonna decide whether I'll be going to university. Does anyone have a inkling to what topics may come up by looking at past papers? I heard that everything on the spec has to be covered over 3 years of exams?

[Z REFAN Z]

Anyone can post any material besides past papers and answers please

[ds4143]

(Original post by otrivine)
thanks where did you go ? answer faster

ok
Describe the process of electrophoresis (4)

Sorry! I didnt realise you replied
Okay umm DNA is mixed with restriction enzymes which cut them into bits. It is also mixed with radioactive markers.
The sample are placed into wells at the negative side of the gel.
Gel with samples inside wells is place in a solution and an electric current is passed through.
Because dna is negatively charged it moves towards the positive electrode. The different lengths of dna samples moves at different speeds...shorter ones travel faster than longer ones.
The radioactive markers help us see where the dna samples has reached.

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[otrivine]

(Original post by ds4143)
Sorry! I didnt realise you replied
Okay umm DNA is mixed with restriction enzymes which cut them into bits. It is also mixed with radioactive markers.
The sample are placed into wells at the negative side of the gel.
Gel with samples inside wells is place in a solution and an electric current is passed through.
Because dna is negatively charged it moves towards the positive electrode. The different lengths of dna samples moves at different speeds...shorter ones travel faster than longer ones.
The radioactive markers help us see where the dna samples has reached.

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yep yep 4/4

[Issy123]

(Original post by katie.lou)
You need to know that the H zone gets shorter, as do the I bands. the Z lines pull towards each other. Then you just need to know the role of ATP (the ratchet mechanism) and role of calcium ions (to attach to troponin which moves trypomyosin). That's obviously shortened but they're the main things I think

Thank you!! Appreciate it

[ds4143]

(Original post by otrivine)
yep yep 4/4

Woo, okay
Name the different parts of the lac operon (3) and what each of them do (3)


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[otrivine]

(Original post by ds4143)
Woo, okay
Name the different parts of the lac operon (3) and what each of them do (3)


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do you mean the binding sites?

[ds4143]

(Original post by otrivine)
do you mean the binding sites?

Nope, well binding site is a part of it, but there are 3 specific named parts of the lac operon that do different things.

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[Sparkzz]

(Original post by otrivine)
in my book page 24 is on F214?????????
are u using a different book cause in the ocr does not mention !?
more please
ok let me give you some as well so did i get 7/10 ?
1)Suggest Name 2 ways to treat sickle cell and what amino acid is replaced (3)
2)what do you understand by the term Restriction enzyme (1)

Sorry page 124.


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[otrivine]

(Original post by ds4143)
Nope, well binding site is a part of it, but there are 3 specific named parts of the lac operon that do different things.

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do you mean Z X Y?

[otrivine]

(Original post by Sparkzz)
Sorry page 124.


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oh yes thanks i will revise that page again can you give me more question

[ds4143]

(Original post by otrivine)
do you mean Z X Y?

Lol sorry im not wording the question right. Im talking about the parts if the operon...the structural region, promoter region and the operator region

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[otrivine]

(Original post by ds4143)
Lol sorry im not wording the question right. Im talking about the parts if the operon...the structural region, promoter region and the operator region

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oh sorry
the functions for promoter region is to bind with RNA polymerase to allow transcription of enzyme B galactosidase and lactose permisrase
Operator region is where the repressor protein binds
Strucutral region is the switiching of genes on or off

[ds4143]

(Original post by otrivine)
oh sorry
the functions for promoter region is to bind with RNA polymerase to allow transcription of enzyme B galactosidase and lactose permisrase
Operator region is where the repressor protein binds
Strucutral region is the switiching of genes on or off

The promoter region is correct, but the other two are slightly wrong ...

Structural gene - codes for enzymes, b-galactosidase and lactose permease
Operator region - switches the structural genes on or off.

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