[callmetom]

At the moment I have to analyze proteins with bio HPLC. But in my opinion our HPLC columns are not suitable for my researches. That´s why i´m searching for columns with a dimension of 150x2.1 . Currently we´re buying Phenomenex SEC columns. But this columns selectivity declines after increasing or decreasing the charge. Where can I get good advice? is it even feasible to try HPLC columns of diverse producers?

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[stanley987]

Hi callmetom,
the HPLC columns with a size of 150x2.1 you can get from any producer doesn´t matter whether to buy from Merck, Agilent, Grace or Thermo. Unfortunately i don´t know the Phenomenex SEC HPLC column, so i can´t help you with changings in selectivity. But i think it´s possible to test a HPLC column. If I remember right I bought uor last HPLC columns at http://www.analytics-shop.com/gb/chromatography/liquid-chromatography-hplc/hplc-columns.html and I could test the columns a few days. Perhaps you should just call them and ask.
Good luck!