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AQA DNA Hybridisation

emma7645

Do I need to know the process of DNA hybridisation for the Unit 2 exam?

Reply 1

Jedidja

Yeah you do, during semi conservative replication

Reply 2

ps1265A

Yes, do you need help understanding it?

Reply 3

ViciousPenguin

If someone could explain it in steps that would be great, will help with your revision too;D

Reply 4

physicsftw

dna from two organisms being studied obtained
dna of one of the organisms radioactively labelled. The other dna is not labelled
dna strands heated to separate the strands
single strands of dna from the two organisms mixed
dna fragments that are 50% labelled are selected(because they consist of one strand from each organism)
dna fragments heated, the temperature at which the two strands separate is noted
the higher the temperature, the more complementary bases between dna of two organisms so the more hydrogen bonds between the two strands
and so the more closely related the two organisms are

Reply 5

physicsftw

Original post by ViciousPenguin

If someone could explain it in steps that would be great, will help with your revision too;D

check my comment in the thread if you want the explanations

Reply 6

ViciousPenguin

Original post by physicsftw

check my comment in the thread if you want the explanations

Thank you! It's nice to read it in simpler points that the text book gives :smile:

Reply 7

ImABigOldTurd

Original post by Jedidja

Yeah you do, during semi conservative replication

I'm afraid you're wrong.

Semi-conservative replication takes part during interphase.

DNA hybridisation is the process whereby scientists can see how similar two strands of DNA from different organisms are.