ok, well you don't need enzymes to decompose H2O2 it decomposes on its own in light or much faaster with Manganese (IV) oxide, although of course these have different activation energies being different processes. I take it that you are referring to a specific decomposition using a specific enzyme?.
Enzymes still operate over a range of temperature just not when the temperature rises too much. Why not carry out the reaction at 20ºC, 25ºC, 30ºC and 35ºC to obtain results from which to calculate activation energy. Use a thermostatically controlled water bath.