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Limitations imposed by the nature of the phospholipid bilayer

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    (Original post by Puddles the Monkey)

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    Cells that are frozen are thawed can be injured due to ice crystals piercing the bilayer. This also deforms the bilayer of organelles. The membrane's permeability to all molecules increases as a result. No control of entry or exit of molecules passing through the bilayer will kill the cell.

    The bilayer is prone to lipid peroxidation. This can rupture the cell membrane and also lead to cell death.

    The bilayer only properly functions within a certain span of temperatures. This isn't really a limitation as the various intracellular enzymes and proteins would denature before the bilayer loses its functionality, so the bilayer itself isn't the limiting factor if its not the first to "give way".

    I'm sure with a little thought one could think of plenty more examples.
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