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Immobilisation of enzymes

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    • Thread Starter

    I was revising biotechnology and I came across a difficult question that my teacher asked me. How could you test for the lactase enzyme that has been lost in a solution of milk? I thought that using the biuret test (the test for protein) would work but realised milk has its own proteins. I know its a silly question but I have been stressing about it.

    Thank you.

    Measure the amount of lactose and glucose at the start, add some more lactose and measure the amount of glucose at the end. If Lactase is present, it would have catalysed the breakdown of lactose into glucose and glucose is easy to measure.
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Updated: April 17, 2016
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