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    • Thread Starter

    Hello there

    I wonder if anyone may please be able to help me?

    If I have made a dilution of a substance by adding 300ul of solute to 300ul solvent and then taking 300ul of this and adding it to a further 300ul of solvent - doing this until I get a dilution of 1:16 (therefore repeating the dilution 4 times), and I want to go from the concentration of the solute at 1:16 back to the original concentration of the solute when it was neat, what should I do? For example, if I find the concentration of the solute at 1:16 to be 0.40mg/ml, how would I know what the concentration was when it was neat (before dilutions) should I not have a sample of the neat version anymore? Do I need to multiply the concentration at 1:16, e.g. the 0.40mg/ml, by something?

    Thank you very much.
    Attached Images

    If the final one was 16x more dilute than the stock solution, then the stock solution must have been 16x more conc. than the diluted one.

    Am I misunderstanding you?
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