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if someone can give me an hint to how to proceed with this exercise I will appreciate that...thanks
Original post by carmenbn
if someone can give me an hint to how to proceed with this exercise I will appreciate that...thanks


Start off by plotting the graph of concentration vs velocity (you can calculate them using the Beer-Lambert law). You should find it to be a curve, and the velocity which the curve approaches can be estimated to be Vmax (i.e. the asymptote). Vmax/2 is Km.

Then plot the lineweaver-burk plot (1/V vs 1/substrate) and see if you can work out how to calculate Vmax and Km (think of the gradient and intercepts).
Reply 2
Thanks...but the major problem is that i'm confused about the units...in the exercise it is asked to change them !

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