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Enzyme Buffer Solutions

Hi,

I'm trying design my Bio project at the moment. I'm going to investigate the catalase enzymes in vegetables. (These catalyse the decomposition of H2O2).
I want to keep the pH at a constant level by using a buffer soln.

Could you suggest some buffers I might use and would be available to the school? Atm I'm thinking of neutral pH, but if you could give me some buffers that I could use for acidic/basic pH's, I'd be grateful. :smile:

My Dad suggest tris with saline soln, but isn't this a strong buffer? :confused:

Anyway, Thanks :smile:

Rep Available :wink:

Avalon

Reply 1

Golden Maverick

acid = ethanoic acid + sodium ethanoate
alkaline = ammonia + ammonium chloride
http://www.chemguide.co.uk/physical/acidbaseeqia/buffers.html
neutral = Tris, formalin
I can't find a list of neutral ones, there don't seem to be many simple ones. hmmm

Reply 2

anchemis

err... you can look up for a pH 7 buffer in a science catalogue... it appears as a yellow solution... cant remember what its called tho

Reply 3

TakemetoAvalon

Golden Maverick

neutral = Tris, formalin
I can't find a list of neutral ones, there don't seem to be many simple ones. hmmm

Are you sure it needs formalin? I thought it was just Tris dissolved on its own.... do you know anywhere I might find the concentrations for particluar concentrations?
1M is about ph 8 I think at 25C.

The site was useful, thanks.

Reply 4

TakemetoAvalon

anchemis

err... you can look up for a pH 7 buffer in a science catalogue... it appears as a yellow solution... cant remember what its called tho

Ammonia buffer possibly?

Reply 5

TakemetoAvalon

*bumps thread to top*

look at me! I want attention :biggrin:

Reply 6

nepali_babu

hey im doin the same project n my teacher has made me use citric acid-sodium phosphate buffer solutions. no idea wt a citric acid is but if u know...u cud use it huh!! good luck neway

TakemetoAvalon