F215 - Revision thread 13th June 2011

i need an A in every exam to get an overall B in the course...but thats neva gonna happen is it? No way, i 've only revised ecosystems yet, not looked at anything else and i've not even touched da revsion for chem exam which is 2 days afta F215 exam!

Haha, I seem to be getting up stupidly early recently, whatever time I go to bed Think it's my brain's way of telling me to get up and revise

Argh I know, it's so frustrating. Nothing sticks What grade are you aiming for?

Guys, you know when the bacterial (E.coli) take up recombinant plasmid, why do are they resistant to the antibiotic Ampilicillin but the other bacteria that have original plasmids which did not take up the recombinant plasmid die?

I need an A, which doesn't seem so likely right now with less than a week til the exam...

Anyone staying up tonight to revise?

i'm totally freeeeeeeeeeaking about this exam - it's so content heavy OMD.
Does anyone know if i got a high A last year and a high A in the course work... the average sort of mark in both A2 modules for an over all B?!!?

thaanks

I am possibly
)

All the old papers, Growth, Development, Reproduction, Application of Genetics, Environmental Biology, Microbiology and Biotechnology, Mammalian Physiology and Synoptic questions papers and mark schemes!

http://www.mediafire.com/?4qw4q5v7zb5pivn

You've got 5 days for Bio5 and almost 7 days for the Chem5...remember the new A2 exams are more concerned with the "application of knowledge in novel contexts!"

hey guys what do you think the chances of the chi squared test, epistasis, and genetic diagrams will come up seeing as it came up in june 2010? I've heard about how ocr are trying to cover all of a topics in f215 within 4 exams (not sure whether this is true or not)

hey thanks for the support, yeh im having to quickly rush thru it all, all i can do now is read throughly thru da revision guides and learn da main stuff as i dont have the time to do da extra stuff. gud luk wid ur revision.
thanks

The BAC stuff is just additional knowledge, it's how the human genome project was worked out if anything

Basically, BAC is to produce multiple copies of DNA fragments, giving a clonal libary - and giving overlapping fragments, because you cut the DNA fragments with restriction enzymes.

Then, it's just the automated sequencing method, otherwise known as the Chain-termination method:

1) DNA fragments are mixed with free DNA nucleotides, DNA primers, DNA polymerase and (flourescently labelled nucleotides, known as terminator bases)
2) The primer anneals(binds) to the 3' end of the DNA strand, so DNA polymerase can attach free DNA nucleotides to it.
3) Then, a terminator base will be attached, and this will throw off DNA polymerase, so the fragment of DNA will stop at a specific place

So now, you use electrophoresis where the fragments of DNA are seperated by there length > the shorter fragments will go through the gel quicker, and also will then reach the end where it will be exposed to UV light, and whatever colour it gives off will be fed into a computer system to show an electrophoresis graph.

Therefore, your then able to work out the colour sequence, which shows one of four colours (for the terminator bases which are flourescently labelled) > and then work out the sequence of nucleotide bases, as the shortest DNA fragment will always have one nucleotide, then another will have two. etcetc.

Hope that helps

Biology Factsheets are very useful are summarising the entire Biology spec...great for the Synoptic element!

http://www.mediafire.com/?ymp31saiz5rqlzs