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F215 - Revision thread 13th June 2011

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Original post by Jhyzone
complementary DNA.



Original post by M_I
What is cDNA?

:frown:


i thought it was a copy of the DNA?
Original post by Jhyzone
YES actually, they can show us a front or back of the brain and label it :biggrin:
YUM! I did psychology anyway so I should know. THat's why I love few chapters from module 4 esp if a question on how the nervous system and endocrine system response to stress, I would nail it!!


Same, aha. I hope the WHOLE exam is on that. Maybe a bit about phenotypes and stuff.

And nothing at all about Biotechnology, because I just don't get it!
Original post by ChubbyRain
i thought it was a copy of the DNA?


Yes it is a complementary copy of the DNA
Reply 2223
Avatar for jagdej
jagdej
can someone please explain why skeletal muscle is often referred to as striated muscle
thank
Original post by atman7
Yeah sure heres the link I uploaded them earlier but got buried in here so help yourself

http://www.megaupload.com/?d=73ALBJ4C

be sure to have taken the one i attached there not in the pack sadly I forgot to put them in


You're a lifesaver!!
Reply 2225
Avatar for Drewus
Drewus
Original post by ChubbyRain
i thought it was a copy of the DNA?


It's the complimentary strand of DNA you get when you treat mRNA with reverse transcriptase :smile:
Reply 2226
Avatar for Humz7
Humz7
1
Original post by Ro27
I have markscheme, PM me e-mail address or tell me how to attach LOL?


no worries I found it off the net.
Reply 2227
Avatar for atman7
atman7
8
Original post by jagdej
can someone please explain why skeletal muscle is often referred to as striated muscle
thank


its just the way it looks like. It looks like it has stripes going down it
what is non reproductive cloning?
Reply 2229
Avatar for katie93
katie93
Original post by rebeccalouise_92
what is non reproductive cloning?


cloning of a few cells/a tissue/an organ not a whole organism
Original post by Jhyzone
Yes it is a complementary copy of the DNA



Original post by Drewus
It's the complimentary strand of DNA you get when you treat mRNA with reverse transcriptase :smile:



ahh okay cool :smile:
Original post by rebeccalouise_92
what is non reproductive cloning?


wen you create an embryonic stem cell that's genetically identical to an organism
Original post by katie93
cloning of a few cells/a tissue/an organ not a whole organism



Original post by rebeccalouise_92
what is non reproductive cloning?



In addition to what katie said:
It's also called therapeutic cloning, so it is mainly used to treat genetic disorders.
And it's mainly used for research purposes as well.
Original post by ChubbyRain
i thought it was a copy of the DNA?


it is copy dna
in all honest opinion do you guys think it will be difficult to get 90 UMS in this exam..
How are genomes sequenced?

1) Multiple copies of genome (all DNA in nucleus) made via PCR.

2) All this DNA is then broken up into small fragments (by passing it through a tiny hole under high pressure)

3) Next step involves making multiple labelled copies of each of these short strands. This involves mixing solution of DNA fragments with DNA polymerase, primers, free nucleotides and free nucleotides that are labelled (fluorescent/radioactive marker).

4) 4 different dyes are used (one for each base)

5) Labelled nucleotides are modified so that when they are added to growing nucleotide chain, it stops any more nucleotides being added.

6) This results in many different lengths of DNA, each ending with a labelled nucleotide.

7) Mixture then separated using gel electrophoresis. Shorter DNA fragments move more quickly.

8) Computer records the colours as they pass the end of the capillary tube.

9) This is done for every piece of DNA and gives the overall sequence of nucleotides in the genome.

Would this be a suitable answer? What could I improve upon or add?
What is reclaiming habitats? :biggrin:
Is it reintroducing similar organisms to a habitat which has been damaged/fragmented?
Some good websites here http://www.biozone.co.uk/links.html

The Biozones OCR books are great...the textbook for some reason over complicates topics! ahhh
Reply 2238
Avatar for -Jaz-
-Jaz-
Original post by CoventryCity
Can someone briefly go through Energy transfer in a ecosystem please?


If you mean the reasons why its never 100% efficient:

Sun to producer
1. Some light reflected off leaf
2. Some light passes through leaf, not hitting chloroplasts
3. Light of wrong wavelength
4. Light absorbed by non-photosynthesising parts, e.g. bark

Producer to Primary consumer
1. Cellulose not digested therefore egested
2. Lost via respiration so never reaches consumer
3. Energy used by microorganisms in gut of consumer
4. Barks not eaten

Primary to secondary
1. Skin, bones, nails not eaten
2. Microorganisms in gut use energy
3. Energy lost as heat/by moving around, etc. so never reaches consumer

Any help? :smile:
Hows everybody feeling for the test?
I'm terrified :/

Especially because I keep forgetting what reproductive cloning is and what non-reproductive cloning is. Can somebody clarify please? Would really appreciate it :smile:

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