Glycolysis
ATP hydrolysed Pi combines with glucose making -glucose6phosphate
Glucose6phospahte
by isomrase enzyme becomes. fructose 6phospahte another ATP molecule is hydrolysed phosphate molecule attaches to C1 molecule and becomes fructose-1,6-bisphosphate
They energy released from the hydrolysed ATP activates the hexose 1,6 bisphosphate molecule not to leave the cell
The hexose 1,6 bisphosphate is broken down into 2 molecules of triose phosphate a 3 C compound
TP is dehydrogenated by enzymes dehydrogenase and H atoms combine with NAD substrate phosphorylation of ADP and Pi
Four enzyme catalysed reactions convert TP into 2 molecules of pyruvate
Substrate level phosphorylation occurs
Net gain of 2 molecules of ATP formed 4 made but used to kick start the process
And 2 reduced NAD
Link reaction
Pyruvate dehydrogenated and decarboxylated h atoms combine with NAD ,reduced NAD
Carboxyl group eventually becomes co2
CoA accepts acetate and carries to kreb cycle
Kreb cycle
CoA releases a state and acetate combines with oxcoloacetate to form
Citric acid (6C)
6C decarboxylated and dehydrogenated co2 And reduced NAD formed 5c molecule formed
5c molecule dehydrogenated and decarboxylated
4c formed changed into another 4c and ATP is phosphorylated
4c changed into another 4c molecule and dehydrogenated FAD is reduced
4c into third 4c and dehydrogenated to regenerate oxcoloacetate
Cycle turns 2 times for each glucose molecule
Chemosmosis and oxidative phosphorylation
Flow of protons to generate ATP chemiosmosis
Reduced NAD and FAD lose H atom and split into H+ and e-
The e- are carried across the etc and release energy as the pass across etc this provides energy for the H+ to flow thought the electron carries and they accumulate and cause and proton gradient
The protons diffuse across the inter membrane space and diffuse into the ATP synthase which drives the rotation part and join ADP and pi
4 Electrons are passed from the last last etc and combine with 4H+ and combine with the final electron acceptor O2 and this produces 2 molecules of water.
2 H atoms from FAD and 2H atoms from NAD
Mitochondria adaptation
Inner membrane folded into cristea for greater S.A for greater respiration
Many ATP synathase in inner membrane to form ATP for oxidative phosphorylation
Matrix has many enzymes and reactants for kreb cycle
Maximum ATP not produce
Some hydrogens leaked thought mitochondrial membrane not enough protons for proton motive force
Some ATP used to actively transport pyruvate into cytoplasm
Some ATP actively transport reduced NAD from glycolysis stage into mitochondria
Anaerobic respiration
Mammals
Pyruvate combines with hydrogen from the oxidised NAD -lactate produced
Enzyme lactate dehydrogenase used called lactase pathway
Oxidised NAD back to glucose to pick hydrogen glucolysis can continue.
Yeast
Pyruvate converted to ethanal from decarboxylation co2 provided
Ethanal combines with hydrogen from reduced NAD to form ethanol and alcohol dehydrogenase catalase reaction
Oxidised NAD back to glucose to pick hydrogen glucolysis can continue.
Respiratory substrate organic substance for respiration
The higher the numbers of hydrogen atoms the higher the relative energy value
More h atoms
More NAD reduced
NAD used in etc
Lipids highest
Proteins
Carbohydrates lowest