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AQA BIOL5 ~ 17th June 2013 ~ A2 Biology

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The gene in question produces a GFP. The gene to be cloned is transplanted into the centre of the GFP gene. Any bacterial cell that has taken up the plasmid with the gene that is to be cloned will not be able to produce GFP and will not fluoresce.
page253

Is this a mistake in the NT textbook?
Surely, if the host cell has taken up the plasmid with gene to be cloned (which has been transplanted into the GFP gene), then GFP protein should be produced?

I think what they mean is if the gene that you want cloned is inserted into the GFP gene (thereby interrupting it), any bacteria that is cloned from it will not fluoresce after as a result of this interruption.

Reply 4521

Alfred1010

Original post by trails

Thanks +1.

What's everyone's progress?
Here's mine:

Learned Unit 5 [ o ]
Learned synoptic elements of all units [ - ]
Essay preparation [ - ]
Unit 5 past papers (June10 and onwards) [ ]
Unit 5 past papers (June09 and before) [ - ]

x = complete
o = in process
- = not happening

(edited 10 years ago)

Reply 4522

Trafficongestion

Please God let the essay be on ATP as that's the only one I'll be preparing for...

Reply 4523

sahjan

I still need to go over a bunch of unit 2 stuff, I hate that this exam is synoptic :frown:

Doing really bad in past papers and dreading the essay.

Reply 4524

Tomatochuckers

Original post by Alfred1010

Edexcelite (oh yes I did) here:

Section 1- Respiration/Muscles (Done)
Section 2- Heart (In Progress)
Section 3- Nervous System (Nearly done)
Section 4- The Brain (Nearly done)
Pre-release revision (not done)
Past papers 2010 onwards (not yet)
Sleep (slept quite a lot during the day)

Reply 4525

Soncheese

Original post by Alfred1010

What grade do you need? :smile:

This was posted from The Student Room's iPhone/iPad App

Reply 4526

trails

Right now I'm just doing questions from previous spec. papers, I only have one more current spec. paper to do, but I wanted to do one on the day. Tomorrow I'll also be looking over my notes from BIOL1/BIOL2/BIOL4 again, followed by re-reading essay plans, then about an hour before go back read over unit 5 topics. Absolutely praying that the essay title is something on protein shapes (this is what my teacher predicts, because proteins pop up everywhere)!

Strangely, I'm not nervous about this exam, although that is subject to change. What is everyone else feeling about it? Good? Bad?

Reply 4527

Alfred1010

Original post by sahjan

I still need to go over a bunch of unit 2 stuff, I hate that this exam is synoptic :frown:

Doing really bad in past papers and dreading the essay.

At least it's only the essay that's synoptic. :frown:

But yeah, your second line applies to me too.

Original post by Tomatochuckers

Nice. Your "Sleep" part will apply to me today. :biggrin:

Original post by Soncheese

What grade do you need? :smile:

This was posted from The Student Room's iPhone/iPad App

I need a B overall for uni. I've been told to aim for a low B in this exam, I'm not sure though.
How about yourself?

Reply 4528

Soncheese

Original post by Alfred1010

At least it's only the essay that's synoptic. :frown:

But yeah, your second line applies to me too.

Nice. Your "Sleep" part will apply to me today. :biggrin:

I need a B overall for uni. I've been told to aim for a low B in this exam, I'm not sure though.
How about yourself?

Good luck! And i only need 70-80ums for uni :smile:

How much time should I spend on the essay? Including the planning

This was posted from The Student Room's iPhone/iPad App

Reply 4529

Alfred1010

Original post by Soncheese

Good luck! And i only need 70-80ums for uni :smile:

How much time should I spend on the essay? Including the planning

This was posted from The Student Room's iPhone/iPad App

It's 40 minutes for the essay leaving you 95 minutes for the questions.

I'd advise 5 minutes (10 minutes maximum) for the plan.

Out of curiosity, do you gain marks for a plan?

Reply 4530

Scienceisgood

Guys, are TAQ polymerase and DNA both used in PCR as in, will the MS accept both?

Reply 4531

sahjan

Original post by Alfred1010

At least it's only the essay that's synoptic. :frown:

But yeah, your second line applies to me too.

I thought the whole paper was synoptic 😕 I've done past papers where some of the answers have been purely unit 2 stuff even though what I said could have been a valid answer :frown:

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Reply 4532

Scienceisgood

Original post by Alfred1010

It's 40 minutes for the essay leaving you 95 minutes for the questions.

I'd advise 5 minutes (10 minutes maximum) for the plan.

Out of curiosity, do you gain marks for a plan?

Yes you do because it shows you intended on talking about it but never got around to it, so, you knew what you were talking about.

Reply 4533

Scienceisgood

Well guys, I am now going to get very little sleep between now and Wednesday. :frown:

Reply 4534

omidsassy

**** i havent slept, should i sleep?!?!

Reply 4535

omidsassy

or is there no point seeing as its morning, if the essay is on ATP i swear im going to buy cigars and hand them out to strangers

Reply 4536

pjanoo

Here's hoping the essay choices are on ATP, enzymes or ions, I swear if I knew an examiner personally I'd give them my life savings right now just to know those essay titles!!