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Enzyme concentration calculation, please help me! D:

3M/L

I need to calculate the concentration of the enzyme in μM (micro molar) from moles?
(edited 6 years ago)
Original post by AryaStark
Firstly
The concentration of the enzyme is 5mM (mili molar)
- 300μl of the enzyme was used
The concentration of the buffer is 0.1M (molar)
- 1.7ml of the buffer was used

I need to calculate the concentration of the enzyme in μM (micro molar) in this solution?

Secondly
The concentration of the enzyme is 5mM (mili molar)
- 300μl of the enzyme was used
The concentration of the buffer is 0.1M (molar)
- 1.2ml of the buffer was used
0.5ml of an inhibitor was added (concentration = 1.5mM)

What was the concentration of the enzyme in this solution in μM (micro molar)?


I have no idea, help would be most appreciated



I would be inclined to convert all dimensions into mol, mol dm-3 and dm3 to do the calculation and then change the answer to the units required ...

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