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HPLC, need help reading chromatograms

I carried out an experiment which resulted in a chromatogram with three peaks, now each peak belongs to one of three drugs. How can I associate each peak with its corrensponding drug? :s-smilie:

I have retention times of the drugs but cannot find any references online, if it helps they are aspirin, caffeine and paracetamol.
Original post by SunTiger
I carried out an experiment which resulted in a chromatogram with three peaks, now each peak belongs to one of three drugs. How can I associate each peak with its corrensponding drug? :s-smilie:

I have retention times of the drugs but cannot find any references online, if it helps they are aspirin, caffeine and paracetamol.


Use their respective polarity( degree of bonding to the stationary phase). The 'stickiest' will be the slowest, i.e. last to appear
Reply 2
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SunTiger
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Original post by charco
Use their respective polarity( degree of bonding to the stationary phase). The 'stickiest' will be the slowest, i.e. last to appear


And how would I do that? This is all I have got...

http://imgur.com/a/9eMLY
Original post by SunTiger
And how would I do that? This is all I have got...

http://imgur.com/a/9eMLY


You must know what the stationary phase is if you run a chromatography column ...

http://lab-training.com/landing/free-hplc-training-programme-6/
(edited 7 years ago)
Reply 4
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SunTiger
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Original post by charco
You must know what the stationary phase is if you run a chromatography column ...

http://lab-training.com/landing/free-hplc-training-programme-6/


I know the phases, stationary, mobile etc... Also the retention time can show which drug is eluted faster, but how can I match the drug to the retention time? Am I missing something? Googleing didn't help much.
Original post by SunTiger
I know the phases, stationary, mobile etc... Also the retention time can show which drug is eluted faster, but how can I match the drug to the retention time? Am I missing something? Googleing didn't help much.


The three drugs do not have the same intermolecular forces. If you used a non-ionic stationary phase then the most polar will elute first, etc.

Compare the structures of the three pharmaceuticals and think about their intermolecular forces.

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