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Calculate the number of yeast cells per cm3

Does anybody know how to calulcate the total number of cells by converting chamber volume to cm^3? I've attached the question

I've tried doing Total Cell Count = Total number cell count x 100 (DF) x Volume of chamber)

ex. the first one has a total cell count of 9 so I did 9 x 100 x (4x10^6) = 3.6x10^9 but it doesn't seem right.
Original post by iNicole14


I've tried doing Total Cell Count = Total number cell count x 100 (DF) x Volume of chamber)

ex. the first one has a total cell count of 9 so I did 9 x 100 x (4x10^6) = 3.6x10^9 but it doesn't seem right.


Shouldn't it be 4x10^-6 when converted to cm3 ? :hmmmm:
(edited 4 years ago)
Reply 2
Oh yes, I didn't realise that :colondollar:, makes much more sense now so that'd make it 1.44x10^-3. I'm not sure if you include (4x10^-6) in the caluclation or just do 3.6 x 100 x 10^-6 to get 3.6x10^-4
Original post by Spannerin'moi
Shouldn't it be 4x10^-6 when converted to cm3 ? :hmmmm:
(edited 4 years ago)
Original post by iNicole14
Oh yes, I didn't realise that :colondollar:, makes much more sense now so that'd make it 1.44x10^-3. I'm not sure if you include (4x10^-6) in the caluclation or just do 3.6 x 100 x 10^-6 to get 3.6x10^-4


no worries..I'm trying to understand as well...shouldn't the dil. factor be 0.01 tho?
Attachment not found
(edited 4 years ago)
Reply 4
I did the calculation based on the method done by these two websites https://www.asbcnet.org/lab/getstarted/Documents/Yeast-4.pdf on page 2 and https://www.whitelabs.com/beer/cell-counting-viability-testing

They kept the dilution factor of 10 and 200 the same in the calculation so I didn't change it so I don't know if I should change the DF factor or not.

(I can't see your attachment btw)
Reply 5
It's gotta be at least 10.
Reply 6
What's got to be at least 10?
Original post by Thog
It's gotta be at least 10.
Reply 7
Original post by iNicole14
What's got to be at least 10?

The number of yeast cells per cm^3
Original post by iNicole14
I did the calculation based on the method done by these two websites https://www.asbcnet.org/lab/getstarted/Documents/Yeast-4.pdf on page 2 and https://www.whitelabs.com/beer/cell-counting-viability-testing

They kept the dilution factor of 10 and 200 the same in the calculation so I didn't change it so I don't know if I should change the DF factor or not.

(I can't see your attachment btw)

I see...
what do you think of this link?
https://www.hemocytometer.org/dilution-factor/
uploaded the attachment

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