Another tricky one...method anyone please?
Hydrolysis of ATP is catalysed by the enzyme ATP hydrolase. box
A student investigated the effect of ATP concentration on the activity of ATP
hydrolase. She used shortening of strips of muscle tissue caused by contraction as
evidence that ATP was being hydrolysed.
• She took four slides A, B, C and D, and added strips of muscle tissue of the same
length to each slide.
• She then added the same volume of ATP solutions of different concentrations to the
four slides and left each slide for five minutes.
• She then recorded the final length of each strip of muscle tissue.
Her results can be seen in Table 1.
Table 1
Slide Concentration of ATP solution added to slide /
×10 6 mol dm 3
A 2
B 4
C 6
D 8
0 8 . 5 The hydrolysis of 1 dm3 of a 1 mol dm−3 solution of ATP releases 30 500 J of energy.
60% of the energy released during the hydrolysis of 1 mol dm−3 of ATP is released as
heat; the rest is used for muscle contraction.
The student added 0.05 cm3 of ATP solution to slide D.
Calculate the energy available from ATP for contraction of the muscle on this slide.
3 marks