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Help with enzyme kenetic

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Reply 20

Reality Check

Original post by Xcyx336

According to the graph, Km with inhibition should be 30 right?

LIH has given you a good explanation there :smile:

Reply 21

username4648086

Original post by Xcyx336

image-1bbc1c27-a0ba-4bee-8ce4-2887808919f71028505900112863108-compressed.jpg.jpeg

Ref :bbc

22F94AB7-D7CD-4287-8A10-A0A4293A0B7E.jpg.jpeg

Reply 22

Xcyx336

Original post by lyer_in_hellfyre

Ref :bbc

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Reply 23

Xcyx336

Original post by lyer_in_hellfyre

Ref :bbc

Think now are correct

Reply 24

username4648086

Original post by Xcyx336

Think now are correct

Original post by Xcyx336

According to the graph, Km with inhibition should be 30 right?

Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....

(edited 4 years ago)

Reply 25

Xcyx336

Original post by lyer_in_hellfyre

I thought the question was asking abt Kmapp?

Question itself is asking about two Km one is absence of inhibition and one is presence of inhibition and finally it's asking for Ki

Reply 26

Xcyx336

Original post by lyer_in_hellfyre

Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....

Hold on. We are now sure that Km without inhibition is definitely 10 and with inhibition is 30 according to the graph

Reply 27

username4648086

Original post by Xcyx336

Question itself is asking about two Km one is absence of inhibition and one is presence of inhibition and finally it's asking for Ki

Yep so now we have got values for Km, Kmapp, concentration of inhibitor...now just plug in to this equation
7C2EFB10-2F96-4C23-BA4B-E43A89F3D9D9.jpg.jpeg

7C2EFB10-2F96-4C23-BA4B-E43A89F3D9D9.jpg.jpeg

Reply 28

Xcyx336

Original post by lyer_in_hellfyre

Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....

But for Ki we have to know Kmapp first. The Kmapp should be 33?

Reply 29

Xcyx336

Original post by lyer_in_hellfyre

Yep so now we have got values for Km, Kmapp, concentration of inhibitor...now just plug in to this equation
7C2EFB10-2F96-4C23-BA4B-E43A89F3D9D9.jpg.jpeg

How do you know the Kmapp is 30?