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helpme456
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#2661
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#2661
(Original post by Minnie me)
It's not positive feedback as once the of ovaries have been stimulated to release oestrogen, the stimulation being FSH. The oestrogen oestrogen which is released inhibits the release of FSH, this keeping the FSH concentration low to ensure no more follicles develop, thus this process is an example of negative feedback.

Hope you understand

Me.
(Original post by currydud)
FSH stimulates release of oestrogen BUT then oestrogen inhibits FSH.
if FSH stimulated the release of oestrogen and oestrogen stimulated the release of FSH and so on, this would be positive feedback
OK thanks guys
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Minnie me
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#2662
(Original post by helpme456)
OK thanks guys

your welcome.
Me
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currydud
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#2663
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#2663
(Original post by MLogan)
Its okay but lets hope i am right
fluorescent markers can be used in two ways, one way is the way you said and it can be used to show that the plasmid containing the desired has been taken up, when using this idea, the desired gene isnt placed in between the GFP gene and is placed somewhere else.
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omidsassy
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#2664
im stressing so hard for this, had 78 in bio 4, i need a C/upperD and i can get an A, how hard is it to get a C on this?! reply would be appreciated guys..i need some reassurance
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omidsassy
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#2665
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#2665
i mean get a B***
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Fergy94
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Can someone explain Oestrogen and transcriptional factors to me please?
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DELETED ACCOUNT
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#2667
(Original post by MLogan)
Its okay but lets hope i am right
Well it really depends what you are using it for

If you're incorporating the gene in a substance you need, then the desired gene will fluoresce.
If you're incorporating the gene in a substance you don't need, then the desired gene will not fluoresce.

A Bio5 question can go both ways.
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wrnicholls
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#2668
(Original post by omidsassy)
im stressing so hard for this, had 78 in bio 4, i need a C/upperD and i can get an A, how hard is it to get a C on this?! reply would be appreciated guys..i need some reassurance
You need reassurance when you have to get a D ?
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miketree
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(Original post by omidsassy)
i mean get a B***
Do a paper in timed exam conditions, including essay, mark harshly, this will give you an indication.
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Dedicated1
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(Original post by Fergy94)
Can someone explain Oestrogen and transcriptional factors to me please?
Oestrogen has a complementary shape to binding sites on transcriptional factors found in the cytoplasm of cells. When oestrogen diffuses into cells, it binds to receptors on transcriptional factors which causes it to change shape which means the binding site to DNA on the same transcriptional factor is exposed. Therefore the activated transcriptional factor diffuses into the nucleus and binds to DNA on the promoter region which allows DNA Polymerase to carry out transcription and the formation of pre-mRNA.
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Fergy94
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(Original post by Dedicated1)
Oestrogen has a complementary shape to binding sites on transcriptional factors found in the cytoplasm of cells. When oestrogen diffuses into cells, it binds to receptors on transcriptional factors which causes it to change shape which means the binding site to DNA on the same transcriptional factor is exposed. Therefore the activated transcriptional factor diffuses into the nucleus and binds to DNA on the promoter region which allows DNA Polymerase to carry out transcription and the formation of pre-mRNA.
Can Oestrogen inhibit transcription aswell?
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Dedicated1
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#2672
Is restriction mapping only carried out on bacteria since it involves cutting up a plasmid with a variety of different restriction enzymes and then sequencing it?
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lifeisgood2012
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If an essay question came up on the following topics what would you write about?

1) importance of proteins
2) Importance of carbohydrates
3) Importance of ATP
4)Importance of water?
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Dedicated1
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#2674
(Original post by Fergy94)
Can Oestrogen inhibit transcription aswell?
I don't think so. I guess it can only be inhibited by a lack of oestrogen in your blood, so less diffuses into cells hence less transcriptional factors are activated.
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F1's Finest
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(Original post by Fergy94)
Can Oestrogen inhibit transcription aswell?

(Original post by Dedicated1)
I don't think so. I guess it can only be inhibited by a lack of oestrogen in your blood, so less diffuses into cells hence less transcriptional factors are activated.
Incorrect.

Oestrogen can act as a repressor or activator.

IT all depends on the on the type of the target cell and the target gene.

The question in the exam will tell you which one of the two, oestrogen acts as.
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rommy123
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#2676
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#2676
really stuck with DNA technology, wondering if anyone has any good notes on so i can understand it better? Thanks in advance
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Dedicated1
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#2677
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#2677
(Original post by Dedicated1)
Is restriction mapping only carried out on bacteria since it involves cutting up a plasmid with a variety of different restriction enzymes and then sequencing it?
Can any one answer this?
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mj.x
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#2678
any predictions for the synoptic essay? =)
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starfish232
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I have some questions about the June 2012 paper:
1. Q3b9(ii)- The mark scheme says 'Ovulation prevented /
egg/ovum not released' and 'Follicle not stimulated /
ripened / does not grow;' - I wrote what FSH and LH do and then wrote that they wouldn't be able to do this as a result of the progesterone. - would I still get the 2 marks rewarded for this?
2. Q6b9i0 - the answer i wrote was that 'food may be eaten causing increase in blood glucose concentration so not measured at normal concentration higher than normal' - would this get a mark?

http://filestore.aqa.org.uk/subjects...W-MS-JUN12.PDF
http://web.alfretongrange.derbyshire...5-QP-Jun12.pdf
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DELETED ACCOUNT
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(Original post by lifeisgood2012)
If an essay question came up on the following topics what would you write about?

1) importance of proteins
2) Importance of carbohydrates
3) Importance of ATP
4)Importance of water?
Importance of proteins
Spoiler:
Show

Vaccinations and immunity (Antibody-antigen complexes).
Phagocytosis (hydrolytic enzymes break down the pathogen to form a phagocytic vacuole) and how T lymphocytes are involved.
Digestive enzymes- lowers activation energy of the break down of maltose, lactose, sucrose into respective monomers
Monomers can be absorbed. Discuss how they are absorbed? Through carrier proteins (facilitated diffusion), co-transport (glucose-sodium pump)
The importance of rubsico (enzymes are proteins) in the light independant reaction. The protein is located in the stroma
Transcription/ Translation, mutation results in a non-functional protein or a tumour
Histones are proteins which hold the chromosomes in place, DNA wounds around histones etc,
Glycoproteins and how they act as biosensors
Insulin is a protein, its produced by beta cells in the pancreas (islets of Langerhans), lowers glucose levels (glycogenesis)
Glucagon is a protein, increases glucose levels, prduce by alpha cells (glycognolysis, gluconeogenesis)
Actin/ Myosin is a protein filament. The role of actin/myosin in muscle contraction (sliding filament mechanism)
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