Scroll to see replies
•
Fragments of the DNA to be sequenced are produced by the use of four dideoxynucleoside triphosphate (ddNTP)
•
DNA denatured to separate it into two strands
•
In the presence of ample supply of each of the four ddNTP(each marked with a fluorescent marker A,T,C,G), DNA is replicated by DNA polymerase, using a primer to begin the process
•
Low concentration of the ddNTP is used so that it will be added only rarely to the lengthening chain
•
In separate reactions each of the three other ddNTP’s is added to identify strands of DNA
•
Adding the products of all four reactions together produces a set of fragments that end at nucleotides with different bases and differ in length by one nucleotide
•
Fragments of the DNA to be sequenced are produced by the use of four dideoxynucleoside triphosphate (ddNTP)
•
DNA denatured to separate it into two strands
•
In the presence of ample supply of each of the four ddNTP(each marked with a fluorescent marker A,T,C,G), DNA is replicated by DNA polymerase, using a primer to begin the process
•
Low concentration of the ddNTP is used so that it will be added only rarely to the lengthening chain
•
In separate reactions each of the three other ddNTP’s is added to identify strands of DNA
•
Adding the products of all four reactions together produces a set of fragments that end at nucleotides with different bases and differ in length by one nucleotide
•
Fragments of the DNA to be sequenced are modified to throw off DNA polymerase when added to a chain.
•
DNA denatured to separate it into two strands
•
In the presence of modified DNA nucleotides(each marked with a fluorescent marker A,T,C,G), DNA is replicated by DNA polymerase, using a primer to begin the process
•
Produces a set of fragments that end at nucleotides with different bases and differ in length by one nucleotide
•
Fragments run through a machine (like electrophoresis) which uses a laser to identify the colour sequence and therefore the base pair sequence of the DNA
•
Fragments of the DNA to be sequenced are modified to throw off DNA polymerase when added to a chain.
•
DNA denatured to separate it into two strands
•
In the presence of modified DNA nucleotides(each marked with a fluorescent marker A,T,C,G), DNA is replicated by DNA polymerase, using a primer to begin the process
•
Produces a set of fragments that end at nucleotides with different bases and differ in length by one nucleotide
•
Fragments run through a machine (like electrophoresis) which uses a laser to identify the colour sequence and therefore the base pair sequence of the DNA
Last reply 3 minutes ago
Official London School of Economics and Political Science 2024 Applicant ThreadLast reply 4 minutes ago
Official: University of Manchester A106 2024 Entry Applicants1291
Last reply 5 minutes ago
Rishi Sunak pledges to remove benefits for people not taking jobs after 12 monthsLast reply 5 minutes ago
JK Rowling in ‘arrest me’ challenge over hate crime lawLast reply 12 minutes ago
year 13 gyg journal : trying not to become an academic victim 🤡📖Last reply 14 minutes ago
NICS Staff Officer and Deputy Principal recruitment 2022 2023Last reply 16 minutes ago
Official: University of Bristol A100 2024 Entry ApplicantsLast reply 18 minutes ago
Can I do medicine with these gcse grades and what unis would be most likely to acceptLast reply 23 hours ago
OCR A-Level Biology A Paper 1 (H420/01) - 5th June 2024 [Exam Chat]Last reply 1 day ago
AQA GCSE Biology Paper 1 (Higher Combined) 8464/1H - 10th May 2024 [Exam Chat]Last reply 3 days ago
AQA GCSE Biology Paper 1 Triple Higher Tier 10th May 2024 [Exam Chat]Last reply 3 days ago
Edexcel A-level Salter's-Nuffield Advanced Biology Papers 1, 2, 3 (9BN0 01-03)Last reply 1 week ago
Unofficial Mark scheme: AQA GCSE Biology Paper 1 Triple Higher Tier 16th May 2023Last reply 2 weeks ago
Edexcel A-level Biology A (Salters-Nuffield) Paper 3 - 19th June 2024 [Exam Chat]Posted 2 weeks ago
Edexcel GCSE Combined Science Paper 1 Biology 1 Higher - 10th May 2024 [Exam Chat]Last reply 3 weeks ago
OCR A-Level Biology A Paper 2 (H420/02) - 14th June 2024 [Exam Chat]Last reply 3 weeks ago
OCR A-Level Biology A Paper 3 (H420/03) - 19th June 2024 [Exam Chat]Last reply 3 months ago
AQA GCSE Biology Paper 2 (Higher Tier Combined) 8464/2H - 9th June 2023 [Exam Chat]Last reply 3 months ago
Unofficial Mark scheme: AQA GCSE Biology Higher Combined 8464/1H 16th May 2023Last reply 5 months ago
AQA GCSE Biology Paper 1 (Higher Combined) 8464/1H - 16th May 2023 [Exam Chat]Last reply 5 months ago
Edexcel GCSE Biology Paper 1 Higher Combined 1SC0 1BH - 16th May 2023 [Exam Chat]Last reply 9 months ago
Edexcel GCSE Biology Paper 2 Higher Combined 1SC0 2BH - 9th Jun 2023 [Exam Chat]Last reply 10 months ago
Edexcel A-level Biology B Paper 3 (9BI0 03) - 21st June 2023 [Exam Chat]Last reply 23 hours ago
OCR A-Level Biology A Paper 1 (H420/01) - 5th June 2024 [Exam Chat]Last reply 1 day ago
AQA GCSE Biology Paper 1 (Higher Combined) 8464/1H - 10th May 2024 [Exam Chat]Last reply 3 days ago
AQA GCSE Biology Paper 1 Triple Higher Tier 10th May 2024 [Exam Chat]Last reply 3 days ago
Edexcel A-level Salter's-Nuffield Advanced Biology Papers 1, 2, 3 (9BN0 01-03)Last reply 1 week ago
Unofficial Mark scheme: AQA GCSE Biology Paper 1 Triple Higher Tier 16th May 2023Last reply 2 weeks ago
Edexcel A-level Biology A (Salters-Nuffield) Paper 3 - 19th June 2024 [Exam Chat]Posted 2 weeks ago
Edexcel GCSE Combined Science Paper 1 Biology 1 Higher - 10th May 2024 [Exam Chat]Last reply 3 weeks ago
OCR A-Level Biology A Paper 2 (H420/02) - 14th June 2024 [Exam Chat]Last reply 3 weeks ago
OCR A-Level Biology A Paper 3 (H420/03) - 19th June 2024 [Exam Chat]Last reply 3 months ago
AQA GCSE Biology Paper 2 (Higher Tier Combined) 8464/2H - 9th June 2023 [Exam Chat]Last reply 3 months ago
Unofficial Mark scheme: AQA GCSE Biology Higher Combined 8464/1H 16th May 2023Last reply 5 months ago
AQA GCSE Biology Paper 1 (Higher Combined) 8464/1H - 16th May 2023 [Exam Chat]Last reply 5 months ago
Edexcel GCSE Biology Paper 1 Higher Combined 1SC0 1BH - 16th May 2023 [Exam Chat]Last reply 9 months ago
Edexcel GCSE Biology Paper 2 Higher Combined 1SC0 2BH - 9th Jun 2023 [Exam Chat]Last reply 10 months ago
Edexcel A-level Biology B Paper 3 (9BI0 03) - 21st June 2023 [Exam Chat]