what is a MINISATELLITE

this is part of an intron structure, apparently its the most important thing in distinguihing between 2 different DNA samples cos everyone has different minisatellite.
Does anyone have a clear defininition of what is , please help!!!!!!!!

Reply 1

Kiesha

10

Google: "A DNA sequence of about 20 to 50 nucleotides which are tandemly repeated from about 5 to 100 times."

Reply 2

Setup

In the context of AS biology you make minisatellites by treating DNA with restriction endonuclease and you have to make sure your minisatellite is there (i.e. your enzyme doesn't break your gene sequence). You then use it for genetic finger printing.

This is my understanding anyway...

Reply 3

oxymoron

7

Setup

In the context of AS biology you make minisatellites by treating DNA with restriction endonuclease and you have to make sure your minisatellite is there (i.e. your enzyme doesn't break your gene sequence). You then use it for genetic finger printing.

This is my understanding anyway...

You don't "make" a minisatellite. The minisatellite is the sequence of DNA itself which everyone has in their genome. What you are refering to is amplifying (or making more copies of) the minisatellite sequence so you can analyse it to find out how long it is.

what is a MINISATELLITE

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