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    I've been doing essays for each of my subjects all day, and I really can't think right now - one of the questions for the Biology write-up I have to do is "Why did you shake the test-tube – how could this affect the enzyme?". The experiment was where the protease - bromelain - hydrolysed gelatine on a cellophane strip after which, we measured the absorbency of the solution in a colorimeter. I've put:

    "It was necessary to shake the test-tube to make sure that the enzyme was hydrolysing all the contents of the solution, and not just small parts of it. Left undone, this again would have affected the readings from the colorimeter."
    is that right? Something doesn't seem correct.
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    Well, you need to shake the test tube so make sure the solution is evenly dissolved. When you are hydrolyzing stuff , the particles remain in that one region and diffuse very slowly. So your Absorbance reading wont be accurate if your measuring the clumps rather than evenly distributed. By shaking it, you evenly distribute all the particles.
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    Well the common sense approach would indicate that shaking the test tube will: cause the substrate to collide more with the active site of the enzyme, thus increase the rate of the reaction as the active site is more on average exposed to substrate.
 
 
 
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