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    Do you remove the tumour inducing gene from the plasmid?
    Got contradicting views on this from the books.
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    yeh...

    cut the gene you want from DNA using restriction endonuclease

    using restriction endonuclease cut out the tumour forming gene from agrobacterium tumefaciens

    use DNA ligase to join the gene to the plasmid (forms recombinant DNA)

    culture the bacterium in a solution with the recombinant DNA

    test for a marker gene (e.g. for antibiotic resistance) to check whether the recombinant DNA is placed into the plasmid

    as the agrobacterium tumefaciens is taken up by plant it causes the useful gene (as it would have done if the tumour gene was present) to be placed into the DNA of the plant

    the useful gene is then expressed... which creates your GM crop

    useful as they may be resistant to hebicides, pestidicide...etc
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    ok safe. thanks a lot. good luck.
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    cheers, good luck!
 
 
 
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