AS Biology Coursework - Beetroot experiment

Hey, i am doing an experiment to test the affect that temperature has on the plasma membrane using a colourimeter. I have done the experiment and now in the process of writing it up and i need a detailed explanition of why this happens and what happens to the plasma membrane as the temperature increases.

Has anyone got any ideas or any websites to go to?
Thanks

Reply 1

Unregistered

OP

An investigation in to the effect of temperature on the release of pigment from beet root tissue - http://www.coursework.info/i/11301.html

Reply 2

bnash

HI there. I had to do the same exact thing. this is my interpretation for it, hope it helps!!

The overall trend seems to be that as the temperature increases, the average percentage of transmission decreases. This is suggesting that membrane permeability increases with temperature and therefore more colour leaks out. The darker the colour means that more pigments have leaked out and therefore the lower percentage of transmission.

From 0ºC to 20ºC, there’s a slight increase in the average percentage of transmission. This is because when water freezes, it expands and this makes the cell membrane bursts. The bursting results in more pigments leaking out and the darker the colour of the liquid obtained.

Between 20ºC and 60ºC, proteins are denaturing more and more as the temperature increases. This is lowering the percentage of transmission as more pigments are leaking out due to the denatured proteins and the lower the transmission (the darker the colour). This is shown in the graph as gradual decrease in transmission.

By 80ºC most of the proteins have already denatured and this explains the steep slope on the graph. By that temperature, most colour has already leaked out and therefore resulting in the highest transmission.

email me if this helps or if you need more!
take care
bnash

Reply 3

Unregistered

OP

does any one know the table of results for the beetroot experiment ' effect of temperature on enzyme function' thx

Reply 4

Xenon

11

Oh yeah, I hated that experiment. For all the time we were doing the practical experiment, we all came out with beetroot red hands. Not very nice!
Oh, sorry can't remember much else about that experiment; Sorry!

Reply 5

xeVeLyNx

SaZ2o1o

does any one know the table of results for the beetroot experiment ' effect of temperature on enzyme function' thx

yea im stuck on that 2! ive just started the planning stage n we need to do some table of variables! does anyone have any ideas of what to include?! thanx it would be really helpful for any suggestions :biggrin:

Reply 6

hornblower

6

Unregistered

Hey, i am doing an experiment to test the affect that temperature has on the plasma membrane using a colourimeter. I have done the experiment and now in the process of writing it up and i need a detailed explanition of why this happens and what happens to the plasma membrane as the temperature increases.

Has anyone got any ideas or any websites to go to?
Thanks

Oh come on! You must've covered cell membranes and the denaturing of enzymes already - this is textbook stuff! Where's the honour asking to be spoonfed?

I did this investigation last year. Playing with beetroot can be harmful to your clothing.

J.

Reply 7

Fairy B

Hiya! I'm also doing the beetroot coursework, and im on the evaluating, which has never been my strong point. I was hoping if anyone would be able to give me any tips as to what to put in the evaluation. Any help would be brilliant. Also, would anyone be able to tell me what the graph should look like if light absorbance is on the y axis??

:smile:

Thankyou! Loadsa love a fairyx

Reply 8

GH

13

The graph should look like what your data shows. You should not try to change the data.

Evaluation: Try to say how the experiment's resul;ts were either sufficiently good enough to support your hypothesis or not.

AS Biology Coursework - Beetroot experiment

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