Dissolve lyophilized enzyme or dilute enzyme suspension to 1.0 mg/ml in 5 mM glycine buffer, pH 8.0. Care should be taken not to shake the solution as precipitation may result. Dilute further immediately before use in 5 mM glycine buffer, containing 0.1% albumin, to obtain a rate of 0.02 - 0.045 ΔA/min.
mgP/ml = A280/min*0.87
The above is from the worthington manual for G6PD assay...
Can some body help me out with this.....
confused whether i should take it at 280nm or 340 nm
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