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    (Original post by logic123)
    So just to clarify im getting it right

    1. The first Eco makes a cut in the 2500 plasmid forming a long strang at 2500bp

    2 Eco then cuts at another site on this 2500 strand to form two strands

    3 The Xal then cuts on of these two strands into another two seperate strands to give me 3 strands totsl?
    Mmm..not quite. The new plasmid is 5000Bp long.

    One fragment is the original plasmid (2500) with Eco cutting at the start and end of it.

    The other 2500 fragment it the inserted gene which Xbal cuts in two.
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