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calculating the purity of DNA Watch

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    Hi

    does anyone know how to assess the purity of the DNA sample, the 260/280 ratio for pure DNA is 1.8- 2.0 ?

    I extracted the DNA and my concentration was ng/ul 45.1 and the optical density was 2.06.

    please help me solve this
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    (Original post by Gamewizard)
    Hi

    does anyone know how to assess the purity of the DNA sample, the 260/280 ratio for pure DNA is 1.8- 2.0 ?

    I extracted the DNA and my concentration was ng/ul 45.1 and the optical density was 2.06.

    please help me solve this
    This may help:http://www.phenogenomics.ca/transgen...ity-Ratios.pdf

    As may this: http://en.wikipedia.org/wiki/Nucleic_acids_analysis

    Both of those are references I used when I was having some DNA extraction and NanoDrop troubles.
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    (Original post by Revd. Mike)
    This may help:http://www.phenogenomics.ca/transgen...ity-Ratios.pdf

    As may this: http://en.wikipedia.org/wiki/Nucleic_acids_analysis

    Both of those are references I used when I was having some DNA extraction and NanoDrop troubles.
    Thank you I will try these websites !
 
 
 
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