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    I want to make a 1/400 dilution of blood in 0.33M Sodium Chloride using two serial 1/20 dilutions.

    How’d I complete this dilution series in micro-tubes starting with 50µl of blood?

    Thanks.
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    What standard chemistry is this?
    Degree level?
    Just wondering out of curiosity
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    (Original post by HKSR)
    What standard chemistry is this?
    Degree level?
    Just wondering out of curiosity
    Yes it's degree level.
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    (Original post by hollywoodbudgie)
    I want to make a 1/400 dilution of blood in 0.33M Sodium Chloride using two serial 1/20 dilutions.

    How’d I complete this dilution series in micro-tubes starting with 50µl of blood?

    Thanks.
    How much do you need and do you need to use all of the blood? If so:

    Take your 50µl of blood and add 950µl 0.33M sodium chloride solution.

    Extract a 50µl this sodium chloride solution and blood mixture. To this extract add another 950µl of sodium chloride solution.

    Result: 1/400 dilution of blood in a 0.33M sodium chloride solution.
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    (Original post by js374)
    How much do you need and do you need to use all of the blood? If so:

    Take your 50µl of blood and add 950µl 0.33M sodium chloride solution.

    Extract a 50µl this sodium chloride solution and blood mixture. To this extract add another 950µl of sodium chloride solution.

    Result: 1/400 dilution of blood in a 0.33M sodium chloride solution.
    You've been very helpful to me, but I'm really lost here. Where'd the 950 come from? :confused:
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    (Original post by hollywoodbudgie)
    You've been very helpful to me, but I'm really lost here. Where'd the 950 come from? :confused:
    You want to dilute 1/20 then 1/20 again to get 1/400.
    1/20 means that for every one part of 'a' you need 19 parts of 'b'.
    As you have 50µl of blood you need 19 times that of sodium chloride solution.
    19*50 = 950
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    (Original post by js374)
    You want to dilute 1/20 then 1/20 again to get 1/400.
    1/20 means that for every one part of 'a' you need 19 parts of 'b'.
    As you have 50µl of blood you need 19 times that of sodium chloride solution.
    19*50 = 950
    :love: Thank you so much!
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    (Original post by js374)
    How much do you need and do you need to use all of the blood? If so:

    Take your 50µl of blood and add 950µl 0.33M sodium chloride solution.

    Extract a 50µl this sodium chloride solution and blood mixture. To this extract add another 950µl of sodium chloride solution.

    Result: 1/400 dilution of blood in a 0.33M sodium chloride solution.
    Hello. Just wondering if you could help me with this question:
    http://www.thestudentroom.co.uk/show...89&postcount=1

    Thanks!
 
 
 
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