Why would the elution volume for proteins of smaller molecular size be larger, compared to that of molecules of larger molecular size ?
Gel Filtration Chromotography Watch
- Thread Starter
- 29-11-2010 14:56
- 03-12-2010 00:04
This is because the larger molecules 'push' there way past the beads/ are unable to enter the pours of the beads, where as smaller molecules enter the pours of the beads meaning they have to travel through the beads before they can leave, giving a large elution volume.
Hope that makes sence? Quote me if it doesn't.