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    This is NOT a thread for people to give information about what is on the exam. This is a thread for revision and advise about the test. I have the written part of the exam tomorrow and I have a few questions about the methodology of the experiment.

    1. Why was everything kept ice cold?
    2. What was the muslin cloth used for?
    3. Why did the choloplats need to be in solution?
    4. What was the centrifuge used for?
    5. Why was an Isolation Medium used?
    6. What were the limting factors in the experiment?
    7. How could these be improved?

    I would really appreciate it if someone could have a go at answering some of these questions.

    Thanks!
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    Or if anyone could think of any more questions that would be great...
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    anyone?
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    I haven't done it yet so I don't know, but the ice cold answer is usually to reduce enzyme activity. Would that fit?
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    1. Why was everything kept ice cold?
    to reduce enzyme activity

    2. What was the muslin cloth used for?
    to ensure only the chloroplasts are filtered into the solution

    3. Why did the choloplats need to be in solution?
    larger surface are for reaction to occur. Temperature fluctuations in solution is less compared to when solid.

    4. What was the centrifuge used for?
    To ensure that the solution only has Chloroplasts

    5. Why was an Isolation Medium used?
    To help seperate the organelles out. The isolation medium can also contain the isotonic solution which means that it prevents the cell bursting or shrinking due to osmotic gain or loss of water.
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    What's the practical part of this ISA?
 
 
 
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