Hey, basically can't work this problem out.
Reacted glycogen phosphorylase with four different cell extracts. I need to work out the enzyme activity and convert it into units of (EU/mg). I know I need to first get the concentration using the absorbance using the equation A=ELC. For example:-
E is molar extinction 6.3x10E3 mE-1 cm-1
so need it in EU/mg
Q.then not sure how to convert into EU/mg
Q.I'm not sure how I work out the enzyme activity once I've got the concentration, help really needed please, I would be so grateful for any suggestions.
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Enzyme activity help needed PLEASE!!!!! watch
- Thread Starter
- 18-03-2011 12:23
- 19-03-2011 17:57
What syllabus is that? :O