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    Hey, basically can't work this problem out.

    Reacted glycogen phosphorylase with four different cell extracts. I need to work out the enzyme activity and convert it into units of (EU/mg). I know I need to first get the concentration using the absorbance using the equation A=ELC. For example:-

    absorbance:- 0.727
    E is molar extinction 6.3x10E3 mE-1 cm-1
    L=1cm

    so need it in EU/mg

    C=A/EL

    Q.then not sure how to convert into EU/mg

    Q.I'm not sure how I work out the enzyme activity once I've got the concentration, help really needed please, I would be so grateful for any suggestions.
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    What syllabus is that? :O
 
 
 
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Updated: March 19, 2011
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