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    Could anyone just tell me what DNA polymerase and the DNA primers roles are in PCR
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    (Original post by CoventryCity)
    Ahh I am OCR for Chemistry.
    Got an A in my January exam, find it way easier than Biology
    I've got OCR F325 Chem on Wed . . . . but have my Maths C3 first thing tomorrow morning and I've just about spent the whole day doing biology - there is just so much to cover.
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    (Original post by atman7)
    Could anyone just tell me what DNA polymerase and the DNA primers roles are in PCR
    Primers they bind to the single stranded dna with complementory base pairing , A with T c with G ... The temperatures at 55 to allow hydrogen bonds to form.

    Dna polymerase is thermophillic it seals the nucleotides and primers at 72 degrees .
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    (Original post by Signed Solution)
    What is in vitro cloning? I think I've confused myself after looking through the internet.
    In vitro is artificially i.e. fertilising egg + sperm in a lab
    In vivo is in a live cell eg a yeast cell/bacteria
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    (Original post by thepwince)
    Just type in hardy weinberg problems into google, there's plenty of examples... and they're all the same format really.
    Why didnt I think of that :/ Thanks
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    Going through revision guides like a boss!!

    Gonna be a late night, but exam ain't till 13.00

    I'll get up at 8am, and read though again on the big essay - esque questions
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    (Original post by atman7)
    Could anyone just tell me what DNA polymerase and the DNA primers roles are in PCR
    At stage 1 of pcr: DNA polymerase, primers and free nucleotides added to reaction mixture and the mixture is heated at a temp of 95 degrees to break hydrogen bonds (DNA HELICASE IN NATURAL DNA REPLICATION)
    - stage 2 involves primers (short single stranded sequences of DNA)annealing to the 3' ends of the seperated DNA strands. DNA polymerase enzyme cannot bind directly to DNA strand, so primers required to DNA polymerase to bind to.
    -Stage 3, mixture heated to 72 degrees (optimum temp for DNA polymerase) and the enzyme extends the primers by adding free nucleotides (like in natural dna rep).

    Once the enzyme reaches the end of the of the dna strand, a new double strand is created.

    -can be repeated many times....exponential growth i.e. amount of dna strands : x2, x4, x8 ect!
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    Sorry if it's been asked before but does anyone have the mark scheme for the Jan 2011 paper it'd be a massive help!
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    how much did you guys revise today for this exam?
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    (Original post by *Funky-buddha*)
    Sorry if it's been asked before but does anyone have the mark scheme for the Jan 2011 paper it'd be a massive help!
    it's on page 12
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    (Original post by kabolin)
    how much did you guys revise today for this exam?
    pretty much all day... i've been revising this for about 10 hours a day for the last week and i still feel like i dont know anything
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    (Original post by kabolin)
    how much did you guys revise today for this exam?
    How many hours have there been since midnight :cool:
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    (Original post by airdoc)
    How many hours have there been since midnight :cool:
    I've done the same as you!

    Doesn't seem "too" bad in fairness. I have a solid idea of what will come up :-)

    I predict these essay questions:

    - How is Insulin produced..( refering to recombinant DNA etcetc )
    - PCR
    - MUSCLES
    - Brain

    I'll get a chinese before my exam
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    I thinnk most people have gone to bed now.
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    (Original post by 786girl)
    what does it mean when it says about apoptosis

    nitric oxide can make inner mitochondrial membrane more permeable to hydrogen ions and dissipating the proton gradient? :sssss
    something to do with more atp perhaps?
    My guess would be something to do with the resulting lower pH being optimal for the enzymes that break down the cell cytoskeleton. Also the protons interfering with hydrogen bonds in the cell.
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    What exactly might come up? can anyone give me a list of what might come up so I could concentrate on that and not waste any valuable time. Thanks
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    (Original post by TobeTheHero)
    What exactly might come up? can anyone give me a list of what might come up so I could concentrate on that and not waste any valuable time. Thanks
    Here don't tell anyone ok...open your textbook and open pages:

    Spoiler:
    Show
    104


    Spoiler:
    Show
    to page 207
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    (Original post by TobeTheHero)
    What exactly might come up? can anyone give me a list of what might come up so I could concentrate on that and not waste any valuable time. Thanks
    Look at the specification.

    Knowing OCR, anything there could come up.
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    31 pages of size 11 font later, I've covered every single point on the spec and i'm still not ready for this exam -.-
    send it to me?
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    (Original post by Wailinza)
    I've got OCR F325 Chem on Wed . . . . but have my Maths C3 first thing tomorrow morning and I've just about spent the whole day doing biology - there is just so much to cover.
    Oh God, I have C3 in the morning too & i'm in the same situation!
    Have not done any maths today at all :|
 
 
 
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