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F215 - Revision thread 13th June 2011

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What does it mean difference between PCR and IN VIVO??
I thought PCR is sequencing DNA whereas in vivo is artificial reproductive cloning?

Reply 2641

Mally-xo

Original post by I'mBadAtMaths

DNA polymerase= synthesises new DNA
RNA polymerase= synthesises mRNA
Taq polymerase= DNA polymerase from thermophilic bacteria; it isn't denatured at high temperatures

thanks! so taq polmerase and DNA polymerase are essentially the same thing and used in PCR right?
whereas RNA polymerase is in transcription?

which is used in the genetic engineering of bacteria like E. Coli? xx

Reply 2642

sportycricketer

Original post by Mally-xo

taq Polymerase is a type of DNA Polymerase that can withstand high temperatures therefore used in PCR.

RNA Polymerase is used Transcription and Lac operon

Reverse Transcriptase and then DNA Polymerase is used in genetic engineering of Insulin :smile:

Reply 2643

CoventryCity

Original post by honoris

What does it mean difference between PCR and IN VIVO??
I thought PCR is sequencing DNA whereas in vivo is artificial reproductive cloning?

For sequencing it is interrupted PCR with the modified nucleotides

PCR is just replicating a piece of DNA over and over again I think

Reply 2644

TobeTheHero

BYEE and good luck everyone :smile:

Reply 2645

sportycricketer

http://www.youtube.com/watch?v=q42I0eY0uYQ

Reply 2646

Mally-xo

Original post by sportycricketer

I LOVE YOU! :teehee:

thanks, good luck in the exam. me and alex (the other guy from scriblink) are gonna go on after the exam to discuss/cry if you wanna join lol xxx

(edited 12 years ago)

Reply 2647

KnuckleheadNinja

WHATS THE DIFFERENCE BETWEEN PCR AND INTERRUPTED PCR????????? omg freaking out now.

**** that was hard

FML

-_- what ******* ever OCR

Reply 2651

Nirgilis

I'll just throw it in there that I could have got anywhere between 40 and 80. We'll just have to wait and see :dontknow:

Reply 2652

slacker07906

Expecting the lowest boundaries ever seen in the history of exams

I loathe OCR.

NOTHING on biotechnology/pcr/electrophoresis/genetic engineering/gene probes/muscles/succession/galapagos/chain termination/golden rice????
and only 2 marks each on cloning and gene therapy? i literally might as well have not revised...

Reply 2655

slacker07906

About 3 people cried in my exam and had to go outside :redface:

Reply 2656

sc0307

Did anyone get around 53% for the percentage question? What did everyone get for the p and q values?

Reply 2657

Nirgilis

Original post by heartskippedabeat

That's what I felt :mad:

.. The test was soo ambiguous IMO :s-smilie:

Reply 2658

heartskippedabeat

Original post by sc0307

Did anyone get around 53% for the percentage question? What did everyone get for the p and q values?

I got 53

I worked it out to be 47, realised it had to be over 50% and just did 1 minus it haha
Not sure i'll get the method mark..

I didn't even get p and q. I should've revised hardy weinberg :redface:

Reply 2659

albag

Original post by sc0307

Did anyone get around 53% for the percentage question? What did everyone get for the p and q values?