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    (Original post by Nadooo)
    well done


    what past papers though? in the book or from the website?
    Thanks.
    Past papers dating back to 01- (of the old spec from freeexampaper site)
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    which past papers have people been using? .. and where can i find them?
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    (Original post by ibysaiyan)
    Yes,primer is few hundred base long which basically tells polymerase enzyme to initiate catalyzation i.e it guides it.
    Probe on the other hand can be radioactive such as in electrophoresis or florescent.
    can you not use a fluorescent marker to mark the DNA bands in the gel?
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    (Original post by Arab_Empress)
    Can anyone answer this?

    Is the 3' end a sticky end?
    or are sticky ends only formed by a DNA is fragmented by restriction enzymes?

    anyone?
    Not sure about the 3' end thing but sticky ends are left by restriction enzyme which leave complementary ends in the vector plasmid,the one we need to know is BamH1 ?
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    (Original post by ibysaiyan)
    Not sure about the 3' end thing but sticky ends are left by restriction enzyme which leave complementary ends in the vector plasmid,the one we need to know is BamH1 ?
    youre scaring me what the hell is BamH1 lol

    Nevermind , I googled it and its restriction endonuclease enzyme, I have heard of that, not sure we need to know the BamH1

    Thanks anywy
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    any one got any hardy weinberg practice questions??
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    (Original post by Arab_Empress)
    youre scaring me what the hell is BamH1 lol

    Nevermind , I googled it and its restriction endonuclease enzyme, I have heard of that, not sure we need to know the BamH1

    Thanks anywy
    You ought to know it,it's a restriction enzyme used in the production of HGH.
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    (Original post by Arab_Empress)
    Can anyone answer this?

    Is the 3' end a sticky end?
    or are sticky ends only formed by a DNA is fragmented by restriction enzymes?

    anyone?
    i think the 2nd option
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    (Original post by ibysaiyan)
    You ought to know it,it's a restriction enzyme used in the production of HGH.
    Are you sure about that?! Theres nothing about it in the spec at all?
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    (Original post by sportycricketer)
    Are you sure about that?! Theres nothing about it in the spec at all?
    The book by mary jones has it besides I am pretty sure that past paper questions have showed up based on it.
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    Theres so much to learn for this exam All the notes for this module go through one eye and out the other
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    (Original post by sportycricketer)
    Are you sure about that?! Theres nothing about it in the spec at all?
    Yeah I havent come across it at all in the OCR book, by Sue Hocking
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    (Original post by ibysaiyan)
    :facepalm: Just do what I have been suggesting forever...
    Lol I have tried so many exam questions and it just doesn't make sense to me. Its almost like my brain has a filter in which it only maintains the information it wants to and eradicates the rest! I can do a genetics question one second, get excited then move onto another question and don't even know what its asking for lol.

    Thanks though
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    If it asks:

    Describe the roles of DNA marker probes? (5 marks). What would you write?
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    (Original post by slacker07906)
    If it asks:

    Describe the roles of DNA marker probes? (5 marks). What would you write?

    DNA marker probes are lengths of DNA used in order to indentify fragments of DNA. They have a complementary shape to the DNA that needs to be indentified, but not always exactly complementary. They often are fluorescent making it easier to identify once they have binded to the other DNA fragment. They are used in processes such as electropheresis.
    Not sure if that is 5 marks though!
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    Can they be used to isolate transformed bacteria from the untransformed bacteria?
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    (Original post by ibysaiyan)
    The book by mary jones has it besides I am pretty sure that past paper questions have showed up based on it.
    How much are you willing to accept to do the exam for me/brain transplant?
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    just finished going through the book 4th time in detail...trust me it really helps
    it helped me all the minor details...

    good luck every1 with your revision
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    (Original post by greenford)
    just finished going through the book 4th time in detail...trust me it really helps
    it helped me all the minor details...

    good luck every1 with your revision
    4th time?! Oh man im still on my first time round and havent even finished yet! Still got most of ecosystems and animal behaviours!
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    (Original post by Killmepls)
    How much are you willing to accept to do the exam for me/brain transplant?
    :rofl:
    1x10^20 dollah,I kid ofcourse =]
    I wouldn't want to sit that dreadful thing again,had enough of it. lol
 
 
 
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