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BCA Assay - Protein calculations

Hi,

In one of my assignments I'm asked to calculate the concentration of protein in different protein standards without drawing a standard curve (even if i were to draw a standard curve i have no reference numbers).

Whilst conducting the practical I obtained their absorbance at (562nm), for example :

Tube 2:
Standard BCA mg/ml (ul) = 20
Water (ul) = 80
Absorbance (562nm) = 0.8555


From only this given information is there a way to calculate protein concentration (mg/ml)?
If so can you please set me some guide lines

thanks :smile:
looks like they want you to apply the beer-lambert law. you must have been told the extinction coefficient of your protein at 562nm somewhere? use A = ecl

but with ONLY the given information you wrote there, i don't think it's possible.

edit: wait, what do you mean by "standard BCA mg/ml"? a 1mg/ml protein standard solution, containing BCA? or just 1mg/ml BCA?
(edited 13 years ago)
i believe it may be 1 mg/ml

There was no extinction coefficient of protein at 562nm given :s-smilie:

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