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    Hello,

    If we have 5' non-blunt ends; can they be marked with the RT technique?

    Thank you
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    By RT are we talking reverse-transcription or real-time here? You'll have to expand on what "marked" means for me I'm afraid
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    Reverse transcricption
    thanks
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    Right I'm a little confused by your question - reverse transcription PCR begins with an RNA molecule which is converted to a cDNA before the usual amplification steps - are you asking whether a dsRNA with 5' sticky ends can undergo this process?

    Sorry, I don't follow.
 
 
 
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