Restriction enzymes: how to identify the sequences they recognized?
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- Thread Starter
- 13-04-2011 02:42
- 13-04-2011 03:25
There are databases listing restriction enzymes and their recognition sequences. Wiki has one- http://en.wikipedia.org/wiki/List_of..._cutting_sites
If you mean how is this done from scratch, then I imagine the same way other protein-DNA interactions (e.g. transcription factor binding) are established - gel shift assays to get a general idea of where recognition sites are, methylation interference to narrow down the individual bases essential for recognition, etc.
- 13-04-2011 13:30
A lot of painstaking labour -_-
- 13-04-2011 13:37
All i know about restriction enzymes.
They are also called endonuclases.
They are specific so only cut pieces of DNA that they recognize...
For example if they cut sequence CCTAGG then a mutation resulting in the sequence CCAAGG will mean that the restriction enyme wont cut it. That principle is used in screening tests to detect disorders.
Where the r.e. doesnt cut is where the mutation is present.
Hope that helps.
- 13-04-2011 13:47
If you have the DNA sequence that you want to cut, there are programmes available where they will identify the cutting sites for you.