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DSL
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#1
Report Thread starter 17 years ago
#1
Hi
We are trying to find some info on lipase digestion with and without bile salts! Has anyone got any ideas?
Thanks DSL xx
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Ralfskini
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Report 17 years ago
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(Original post by DSL)
Hi
We are trying to find some info on lipase digestion with and without bile salts! Has anyone got any ideas?
Thanks DSL xx

If you think of the chemical breakdown of substrate using enzymes as a reaction, and by considering reaction theory, you will realise that the rate of the reaction will be dependant if the surface area of the reactants. Bile salts are responsible for the emulsification of lipids which means they will increase the reactant surface area. This means that the rate of enzyme digestion will increase; possibly I would expect a linear relationship between bile salts concentration and rate of lipid digestion.

Sudan III reagent can be used to test for the presence of fats, however I dont know if it's a quantitative test so may not be suitable.
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s.ahmad3
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Report 17 years ago
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i did this very coursework for AS. basically, its all about surface area. greater surface area more likely collision between enzyme + subsrate, so more enzyme/substrate complexes so faster rate of reaction. bile emulsifies the lipid, increasing surface area. And always remember the control (water in this case).
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DSL
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Report Thread starter 17 years ago
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(Original post by s.ahmad3)
i did this very coursework for AS. basically, its all about surface area. greater surface area more likely collision between enzyme + subsrate, so more enzyme/substrate complexes so faster rate of reaction. bile emulsifies the lipid, increasing surface area. And always remember the control (water in this case).

Thanks!! But what about the method? We are not sure what apparatus to use! How did you do yours? C ya. DSL xx
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