egg albumen practicalWatch
i'm using 2.5mls of each personally, but some people sound like they're usin beakers which may be a better method than test tubes
so what is plotted on the graph? i was thinking about concentration against time, but why am i timeing can anyone help
so do we have to do a graph or what? and how d'ya plot a table coz i dont think there is anythin you can plot?! nightmare
i'll love someone forever if they help me on this
literally somet like "once you have collected the results, plot them on a graph with % denaturation on the y axis and concentration of CuSO4 on the x axis" if you have some preliminary results then just sketch a graph like that and say that the final graph should look like that
jesus, urs is too short?!?! mine's 1800 words and counting i've got to cut 800 words tonight. if you need to fill space then just add more detail to the method for your preliminary work or add more detail to anything really
i am doing this just as a planning experiment, so u dont actually have to do the experiment, just a preliminary one to establish a range that is denatured
all this about colourimeters, opaqueness, is unnescessary just hold a testtube of the albumen against a control one of deionised water, and do it from there
and you dont need 2 draw a graph, as it is just planning
and it has nothing to do with timing, or anything like that you are just seeing what concentration of CuSO4 wont denature the albumen
For the metod just use serial diloutions, 0.01, 0.001, 0.0001, 0.00001. Mix these concentrations with the same amount of egg stuff for each. Leave each tube for 5 minutes and then get reults off a colourimeter. Plot a graph of concentration anlong the x axis and % colour intensity up the y axis. Also record the colour intensity of pure egg albumen to give an end point. When the line on your graph reaches the reading given from the straight egg stuff it is not dentatured.!! Vola
Obviously we have to find out if the albumen is fully denatured. How do we do this?
Someone in my school has mentioned centrifuging...but I'm scared
so using serial dilution wont work surely?