Question One Answer
In the process of Glycolysis which produces a net gain of 2x ATP, two molecules of NAD+ are reduced to NADH. These molecules need to be re-oxidised to NAD+, otherwise Glycolysis would very quickly grind to a halt. In the absence of oxygen to act as the terminal electron acceptor, pyruvate is first decarboxylated and then reduced as the electrons and protons are transferred from NADH to Pyruvate to yield ethanol and NAD+ (catalysed by pyruvate decarboxylase) Hence NAD+ has been regenerated and Glycolysis is free to continue producing ATP from glucose by substrate-level phosphorylation.