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Freeze Fracture

Please may you explain freeze fracture to me and whether it is used for SEM or TEM? The internet contains contrasting explanations!

Also whether cutting & staining is SEM or TEM. Thanks :smile:
Original post by yippdypink
Please may you explain freeze fracture to me and whether it is used for SEM or TEM? The internet contains contrasting explanations!

Also whether cutting & staining is SEM or TEM. Thanks :smile:


This is what I am familiar with:

Freeze fracture is when you dip something in liquid nitrogen (-196C) - it freezes water in the cells instantly, and causes the cell structures to fracture. The cells are then thawed to room temperature under vacuum, so as to avoid the formation of ice crystals.It is then coated in gold or platinum and used for SEM.

http://www.vcbio.science.ru.nl/en/fesem/info/cryosem/

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