Hey there! Sign in to join this conversationNew here? Join for free
x Turn on thread page Beta
    • Thread Starter
    Offline

    2
    ReputationRep:
    Given that virtually all (if not all) bacteria have a version of the restriction-modification system, how do bacteriophages such as T4 manage to infect them? I presume T4 has some mechanism of evading restriction? Does anyone know?
    Offline

    1
    ReputationRep:
    (Original post by nikk)
    Given that virtually all (if not all) bacteria have a version of the restriction-modification system, how do bacteriophages such as T4 manage to infect them? I presume T4 has some mechanism of evading restriction? Does anyone know?
    I think T4 phage contain HMC (hydroxymethylcytosine) instead of C in their DNA to which glucose units can attach and it is this that protects the DNA from degradation.
    I'm not 100% on that though - I'll just have a quick look and see if I can confirm!
    Offline

    1
    ReputationRep:
    (Original post by oxymoron)
    I think T4 phage contain HMC (hydroxymethylcytosine) instead of C in their DNA to which glucose units can attach and it is this that protects the DNA from degradation.
    I'm not 100% on that though - I'll just have a quick look and see if I can confirm!
    Yes ... according to google: :p:

    "Why does the phage T4 replace cytosines in its DNA with glucosylated 5-hydroxymethylcytosine?
    The substitution is done to evade restriction of the viral DNA. DNA containing glucosylated 5-hydroxymethylcytosine is not recognized by the host restriction machinery"
 
 
 
Reply
Submit reply
Turn on thread page Beta
Updated: April 15, 2006
Poll
Do you like carrot cake?

The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd.

Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered Office: International House, Queens Road, Brighton, BN1 3XE

Write a reply...
Reply
Hide
Reputation gems: You get these gems as you gain rep from other members for making good contributions and giving helpful advice.