Join TSR now and get all your revision questions answeredSign up now

Henderson Hasselbach to find ml of solutions Watch

    • Thread Starter
    Offline

    2
    ReputationRep:
    Two 0.1M phosphate solutions are used to make up a phosphate buffer with a pH of 7.45. The pKa for the sodium dihydrogenphosphate, disodium hydrogenphosphate equilibrium is 6.95. How many ml of each solution would you add together to make up a total volume of 500 ml?

    What I've done so far is:

    pH = pKa + log [A-]/[HA]

    so

    7.45=6.95 + log [A-]/[HA]

    7.45-6.95 = 0.5 = log [A-]/[HA]

    10^0.5 = 3.162... = [A-]/[HA].

    [HA]/[A-] = 1/3.162 = 0.316

    It is after this step which I become lost and I am not sure I have done it right up till now anyways. Help would be greatly appreciated.
    Offline

    9
    ReputationRep:
    (Original post by Student21)
    Two 0.1M phosphate solutions are used to make up a phosphate buffer with a pH of 7.45. The pKa for the sodium dihydrogenphosphate, disodium hydrogenphosphate equilibrium is 6.95. How many ml of each solution would you add together to make up a total volume of 500 ml?

    What I've done so far is:

    pH = pKa + log [A-]/[HA]

    so

    7.45=6.95 + log [A-]/[HA]

    7.45-6.95 = 0.5 = log [A-]/[HA]

    10^0.5 = 3.162... = [A-]/[HA].

    [HA]/[A-] = 1/3.162 = 0.316

    It is after this step which I become lost and I am not sure I have done it right up till now anyways. Help would be greatly appreciated.
    Both solutions have the same concentration to start with. What you have obtained here is a ratio. so out of total volume of 500mL,

    volume of HA required = 1/(1+3.162) * 500 mL

    volume of the conjugate base required = 3.162/(1+3.162) * 500 mL

    These two volumes will then add up to 500 mL
 
 
 
Poll
If you won £30,000, which of these would you spend it on?

The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd.

Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered Office: International House, Queens Road, Brighton, BN1 3XE

Quick reply
Reputation gems: You get these gems as you gain rep from other members for making good contributions and giving helpful advice.