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    im so confused how does the genome mapping,shearing link to the electrophoresis, PCR and automated sequencing?

    Am I correct to say genome is mapped, sheared and smaller sections from shearing are placed into BACs which are placed into e.coli cells. Theser are grown in culture to produce clonal libraries and these cells with BAC are cultured and the DNA is extracted from cells and restriction enzymes are used to cut them into fragments.
    Then electrophoresis happens
    then PCR
    then automated sequencing?


    sorry i dont see how it all links


    thaks so much in advance
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    anyone
    im desperate
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    anyone?!? :/ please :/ anything!
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    ??//?
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    PCR is done before gel electrophoresis.
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    (Original post by Dynamo123)
    PCR is done before gel electrophoresis.
    ohhh then where does automated sequencing come in? at the end after gel electrophoresis.
    Textbook should really do it in order :/
    And the BAC-ecoli etc comes before PCR right?

    thank you
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    Yup.
 
 
 
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