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    Hi all,

    Does anyone know why real time PCR will produce a small replicated segment than normal PCR? Had this question in an exam and cannot find the answer in my book or on the internet.

    Thanks
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    (Original post by Scotty Bear)
    Hi all,

    Does anyone know why real time PCR will produce a small replicated segment than normal PCR? Had this question in an exam and cannot find the answer in my book or on the internet.

    Thanks
    I'm sorry but I don't understand the question, could you repeat it word for word from the exam. Also, are you talking about real time PCR or reverse transcriptase PCR?
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    I can't remember the exact wording but the first part of the question was if you have a strand of DNA that is 1500 base pairs long, and you use 2 primers, how big would the sequence generated through PCR be? (I think this first question refers to reverse transcriptase PCR but it wasn't specified). The second part (which I find complete weird) said, in Real time PCR there was 400 base pairs generated, why was there less in RT-PCR than in PCR?
    I thought they would both yield the same amount of base pairs dependant on how big the primer was and as long as there were no problems during replication such as primer dimers.
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    To be honest, I don't think anyone will be able to help you unless you can obtain the exact wording of the question. As far as I remember, quantitative PCR is just a way of quantifying the progress of PCR in real time and shouldn't involve a dramatic change in the DNA product obtained (all other things being equal).
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    (Original post by Ashnard)
    To be honest, I don't think anyone will be able to help you unless you can obtain the exact wording of the question. As far as I remember, quantitative PCR is just a way of quantifying the progress of PCR in real time and shouldn't involve a dramatic change in the DNA product obtained (all other things being equal).
    That's exactly what I thought and what I wrote but I only got 1/8 points on the question 0.o Think I'll definately find my teacher and ask him what the answer is because no-one else understood it nor does anyone who I've asked
 
 
 
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