recombinant dna question june 11!

Watch this thread
TaylaShakespeare
Badges: 0
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#1
Report Thread starter 9 years ago
#1
I'm really struggling to understand this question from a june 2011 paper. I found the mark scheme but don't get how the answers even make sense
could anybody help me to understand it?
Scientists used restriction mapping to investigate some aspects of the base sequence
of an unknown piece of DNA. This piece of DNA was 3 000 base pairs (bp) long.
The scientists took plasmids that had one restriction site for the enzyme Kpn1 and one
restriction site for the enzyme BamH1. They inserted copies of the unknown piece of
DNA into the plasmids. This produced recombinant plasmids.
The diagram shows a recombinant plasmid.









When the scientists digested one of the recombinant plasmids with Kpn1, they obtained two fragments. One fragment was measured as 1 000 bp.
The other fragment was described as "very large".

5 (a) (i) What does this show about the base sequence of the unknown piece of DNA?


5 (a) (ii) One of the fragments that the scientists obtained was described as "very large".
What is represented by this very large fragment?
0
reply
TaylaShakespeare
Badges: 0
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#2
Report Thread starter 9 years ago
#2
http://faculty.plattsburgh.edu/donal...h/Restmap.html this website helped a lot
0
reply
Virolite
Badges: 14
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#3
Report 5 years ago
#3
same tbh
0
reply
X

Quick Reply

Attached files
Write a reply...
Reply
new posts
Back
to top
Latest
My Feed

See more of what you like on
The Student Room

You can personalise what you see on TSR. Tell us a little about yourself to get started.

Personalise

Year 12s - where are you at with making decisions about university?

I’ve chosen my course and my university (18)
31.03%
I’ve chosen my course and shortlisted some universities (22)
37.93%
I’ve chosen my course, but not any universities (2)
3.45%
I’ve chosen my university, but not my course (3)
5.17%
I’ve shortlisted some universities, but not my course (4)
6.9%
I’m starting to consider my university options (7)
12.07%
I haven’t started thinking about university yet (1)
1.72%
I’m not planning on going to university (1)
1.72%

Watched Threads

View All
Latest
My Feed

See more of what you like on
The Student Room

You can personalise what you see on TSR. Tell us a little about yourself to get started.

Personalise