Back
to top
recombinant dna question june 11!
Watch this threadPage 1 of 1
Go to first unread
Skip to page:
TaylaShakespeare
Badges:
0
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#1
I'm really struggling to understand this question from a june 2011 paper. I found the mark scheme but don't get how the answers even make sense
could anybody help me to understand it?
Scientists used restriction mapping to investigate some aspects of the base sequence
of an unknown piece of DNA. This piece of DNA was 3 000 base pairs (bp) long.
The scientists took plasmids that had one restriction site for the enzyme Kpn1 and one
restriction site for the enzyme BamH1. They inserted copies of the unknown piece of
DNA into the plasmids. This produced recombinant plasmids.
The diagram shows a recombinant plasmid.
When the scientists digested one of the recombinant plasmids with Kpn1, they obtained two fragments. One fragment was measured as 1 000 bp.
The other fragment was described as "very large".
5 (a) (i) What does this show about the base sequence of the unknown piece of DNA?
5 (a) (ii) One of the fragments that the scientists obtained was described as "very large".
What is represented by this very large fragment?
could anybody help me to understand it?
Scientists used restriction mapping to investigate some aspects of the base sequence
of an unknown piece of DNA. This piece of DNA was 3 000 base pairs (bp) long.
The scientists took plasmids that had one restriction site for the enzyme Kpn1 and one
restriction site for the enzyme BamH1. They inserted copies of the unknown piece of
DNA into the plasmids. This produced recombinant plasmids.
The diagram shows a recombinant plasmid.
When the scientists digested one of the recombinant plasmids with Kpn1, they obtained two fragments. One fragment was measured as 1 000 bp.
The other fragment was described as "very large".
5 (a) (i) What does this show about the base sequence of the unknown piece of DNA?
5 (a) (ii) One of the fragments that the scientists obtained was described as "very large".
What is represented by this very large fragment?
0
reply
TaylaShakespeare
Badges:
0
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#2
http://faculty.plattsburgh.edu/donal...h/Restmap.html this website helped a lot
0
reply
Virolite
Badges:
14
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#3
X
Page 1 of 1
Go to first unread
Skip to page:
Quick Reply
